Journal: Nature Communications
Article Title: Suppression of gut colonization by multidrug-resistant Escherichia coli clinical isolates through cooperative niche exclusion
doi: 10.1038/s41467-025-61327-7
Figure Lengend Snippet: A Results of competition assay in Biolog® Phenotypic microarray competitive index (CFU/ml E. coli MDR1/ CFU/ml E. coli MR102) and OD 600 of the respective strains of all carbon sources that both strains could utilize. One dot represents data from three independent experiments. B Results of competition assay in Biolog® Phenotypic microarray competitive index ( E. coli MDR1/ E. coli MR102) of all carbon sources that could be utilized by at least one of the strains. One dot represents data from three independent experiments. C Competition assay in MM9 supplemented with 5 g/L of the respective carbon source under aerobic and anaerobic conditions. P values indicate a nonparametric Kruskal-Wallis test * p < 0.05. Mean and SD with one dot representing data from one experiment performed in duplicates or triplicates. D Resulting fecal burden of E. coli MDR1 after different time points of colonization. Geometric mean and SD of two independent experiments with n = 8 (PBS), n = 9 (WT) and n = 10 ( ΔmanA and manA : :manA ) mice per group. E Commensal E. coli MR102 colonization levels at different time points. Geometric mean and SD of two independent experiments withof two independent experiments with n = 8 (PBS), n = 9 (WT) and n = 10 ( ΔmanA and manA : :manA ). F Clearance kinetics of E. coli MDR1 after different time points of colonization (clearance = CFU/g below the detection limit in feces). P -values represent the Log-rank (Mantel-Cox) test with 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: B Results of competition assay in Biolog® Phenotypic microarray competitive index ( E. coli MDR1/ E. coli MR102) of all carbon sources that could be utilized by at least one of the strains.
Techniques: Competitive Binding Assay, Microarray
